Scientific studies (review articles) on the relationship between protein and disease prevention:
One swallow does not make a summer. A famous Dutch saying that could not be any more obvious. Just because one single scientific study about a certain topic makes certain claims, it does not necessarily mean it is true. On the other hand, a review article (a collection of scientific studies on a certain topic) of randomized, placebo-controlled double blind clinical trials (RCTs) will answer the following question:
"Do taking dietary supplements make sense?" Yes for a positive conclusion and no for a negative conclusion.
One swallow does not make a summer. A famous Dutch saying that could not be any more obvious. Just because one single scientific study about a certain topic makes certain claims, it does not necessarily mean it is true. On the other hand, a review article (a collection of scientific studies on a certain topic) of cohort studies or case-control studies will answer the following question:
"Should I change my diet?".
- High protein diets causally have beneficial effect on body weight management
- Branched-chain amino acids supplementation during oncological surgical period may reduce post-operative morbidity from infections and ascites
- Protein supplementation increases lean body mass in adults
- Supplementation with L‐arginine alone increases VO2 max in healthy people
- L-arginine supplements do not reduce diabetes in adults
- 10-12 g/d arginine during 8 weeks improve sport performance
- 1-6g/d taurine supplementation improves human endurance performance
- 0.5 to 6 g/d taurine supplementation reduces total cholesterol and triglyceride in patients with liver dysregulation
- Protein supplementation + exercise increase lower-extremity strength in healthy older Asian adults with sarcopenia
- Malnutrition among older adults living in South-East Asia is 25%
- Higher intakes of total protein reduce all-cause mortality
- Protein intake does not increase prostate cancer
- Moderate plant protein decreases type 2 diabetes mellitus
- 20g plant protein reduce type 2 diabetes
- Animal protein increases risk of type 2 diabetes
- Whey protein supplementation increases lean body mass in adults
- Milk protein supplements + resistance training increase fat-free mass in older adults
- Patients with diabetic kidney disease benefit from <0.8 g protein per kilogram body weight per day
- Subjects with end-stage renal disease benefit from a diet with <5.5 En% protein
- No more than 1.62 g/kg/day dietary protein supplementation augments resistance exercise training induced gains in muscle mass and strength in healthy adults
- Protein quality has significant impact on indices of muscle protein anabolism in young and older adults
- >0.8 g proteins/kg body weight/day reduce hip fracture risk in older adults
- Higher protein intake may increase bone mineral density
- A higher intake of plant protein decreases risk of type 2 diabetes among women
- Multiple micronutrients and protein interventions delivered after 24 months of age have a positive effect on linear growth
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- Protein is the body's main source of nitrogen which accounts for about 16% the weight of protein.
- The body of a 76 kg man contains about 12 kg of protein. Nearly half of this protein is present as skeletal muscle, while other structural tissues such as blood and skin contain about 15%.
Myosin, actin, collagen and haemoglobin account for almost half of the body's total protein content. - 1-1.5 grams of protein per kg body weight is needed to maintain a normal calcium metabolism. A normal calcium metabolism is needed for strong bones.
- Muscle wasting is defined as unintentional loss of body weight (5 to 10%) due to accelerated muscle proteolysis, resulting in loss of body cell mass.
- A healthy diet should contain 45-70 En% carbohydrates, 20-35 En% fats, of which 7-10 En% saturated fats and 10-35 En% proteins.
- An ideal diet to maximize muscle growth:
- 1.2-2.0 g protein per kg body weight per day;
- 44-50 kcal per kg body weight per day;
- 3-4 g leucine per day;
- A resistance weight-training program (85-100% of 1RM, 1-6 reps, 3-6 sessions and 3-5 times per week) of at least 10-12 weeks.
- In general, a high protein diet contains 200-400 g of proteins per day.
- Proteins, like milk proteins in foods can cause allergic reactions.
- Proteins contribute less than 2% of the fuel used during exercise of less than one hour’s duration.
Proteins contribute 5-10% of the fuel used during the final minutes of prolonged exercise (e.g. 3-5 hour’s duration). - The tolerable upper limit intake has been set at 2.1 g per body weight per day.
- Protein quality can be measured by Biological Value (BV) and Protein Digestibility Corrected Amino Acid Score (PDCAAS).
- Biological Value (BV) is a value that measures how well the body can absorb and utilize a protein based on nitrogen retention. The higher the Biological Value of the protein you use, the more nitrogen your body can absorb, use, and retain. As a result, proteins with the highest BV promote the most lean muscle gains.
- Protein Digestibility Corrected Amino Acid Score (PDCAAS) is a method of evaluating the protein quality based on both the amino acid requirements of humans and their ability to digest it.
BV and PCDAAS of selected proteins | ||
BV | PCDAAS | |
Whey Protein Isolates | 159 | 1.0 |
Whey Protein Concentrate | 104 | 1.0 |
Whole Egg | 100 | 1.0 |
Cow’s milk | 91 | 1.0 |
Egg White (albumin) | 88 | 1.0 |
Cottage Cheese | 84 | 1.0 |
Tuna | 83 | - |
Fish | 82 | - |
Beef | 80 | 0.9 |
Chicken | 79 | - |
Soy | 74 | 0.9 |
Chickenpeas | 0.78 | |
Fruit | 0.76 | |
Legumes | 0.70 | |
Casein | 71 | 1.0 |
Cereal | 0.59 | |
Peanuts | 68 | 0.5 |
Yogurt | 68 | - |
Oatmeal | 58 | 0.6 |
Wheat | 54 | 0.4 |
Protein souces | Protein content (g protein/100g prodct) | Amino acid score (compared to the egg) |
Egg | 33 | 100 |
Fish | 61 | 100 |
Beef | 29 | 100 |
Milk (cow’s whole) | 23 | 100 |
Soybeans | 29 | 100 |
Dry beans | 22 | 75 |
Rice | 7 | 62–66 |
Corn | 7 | 47 |
Wheat | 13 | 50 |
Wheat (white flour) | 12 | 36 |
Amino acid composition of food proteins (percentage (%) by weight of amino acid) | |||||||
Protein sources | |||||||
Amino Acid | egg white | beef | chicken | whey | casein | soy | yeast |
alanine | 6.6 | 6.1 | 5.5 | 5.2 | 2.9 | 4.2 | 8.3 |
arginine | 5.6 | 6.5 | 6.0 | 2.5 | 3.7 | 7.5 | 6.5 |
aspartic acid | 8.9 | 9.1 | 8.9 | 10.9 | 6.6 | 11.5 | 9.8 |
cysteine | 2.5 | 1.3 | 1.3 | 2.2 | 0.3 | 1.3 | 1.4 |
glutamic acid | 13.5 | 15.0 | 15.0 | 16.8 | 21.5 | 19.0 | 13.5 |
glycine | 3.6 | 6.1 | 4.9 | 2.2 | 2.1 | 4.1 | 4.8 |
histidine * | 2.2 | 3.2 | 3.1 | 2.0 | 3.0 | 2.6 | 2.6 |
isoleucine * | 6.0 | 4.5 | 5.3 | 6.0 | 5.1 | 4.8 | 5.0 |
leucine * | 8.5 | 8.0 | 7.5 | 9.5 | 9.0 | 8.1 | 7.1 |
lysine * | 6.2 | 8.4 | 8.5 | 8.8 | 3.8 | 6.2 | 6.9 |
methionine * | 3.6 | 2.6 | 2.8 | 1.9 | 2.7 | 1.3 | 1.5 |
phenylalanine * | 6.0 | 3.9 | 4.0 | 2.3 | 5.1 | 5.2 | 4.7 |
proline | 3.8 | 4.8 | 4.1 | 6.6 | 10.7 | 5.1 | 4.0 |
serine | 7.3 | 3.9 | 3.4 | 5.4 | 5.6 | 5.2 | 5.1 |
threonine * | 4.4 | 4.0 | 4.2 | 6.9 | 4.3 | 3.8 | 5.8 |
tryptophan * | 1.4 | 0.7 | 1.2 | 2.2 | 1.3 | 1.3 | 1.6 |
tyrosine | 2.7 | 3.2 | 3.4 | 2.7 | 5.6 | 3.8 | 5.0 |
valine * | 7.0 | 5.0 | 5.0 | 6.0 | 6.6 | 5.0 | 6.2 |
* Essential amino acids |